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I used a 1.5mm thickness gel for western blot at 100v for 90min, but the transfer did not complete. Is it necessary to increase the time for an effective transfer?
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Can anyone assist with a transfer issue with li-cor western blot?
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How to keep the voltage stable during a wet transfer of proteins in a Western Blot?
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Western Blot transfer issues (uneven transfer)?
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SDS gel proteins not transferring well to Western Blot PVDF membrane, please help?
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