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I am getting non-specific bands in my western blot and a slightly intense  band of my protein of interest. What should i do?
I am getting non-specific bands in my western blot and a slightly intense band of my protein of interest. What should i do?

Frontiers | Non-specific Detection of a Major Western Blotting Band in  Human Brain Homogenates by a Multitude of Amyloid Precursor Protein  Antibodies | Aging Neuroscience
Frontiers | Non-specific Detection of a Major Western Blotting Band in Human Brain Homogenates by a Multitude of Amyloid Precursor Protein Antibodies | Aging Neuroscience

Western Blot Doctor™ — Protein Band Size and Pattern Problems | Bio-Rad  Laboratories
Western Blot Doctor™ — Protein Band Size and Pattern Problems | Bio-Rad Laboratories

Western Blot Troubleshooting Nonspecific Bands | Sino Biological
Western Blot Troubleshooting Nonspecific Bands | Sino Biological

Western Blot Troubleshooting - Tips & Tricks | StressMarq
Western Blot Troubleshooting - Tips & Tricks | StressMarq

Western Blot Doctor™ — Protein Band Size and Pattern Problems | Bio-Rad  Laboratories
Western Blot Doctor™ — Protein Band Size and Pattern Problems | Bio-Rad Laboratories

Troubleshooting tips for western blotting | Troubleshooting Tips | Support  | Signalway Antibody | Signalway Antibody
Troubleshooting tips for western blotting | Troubleshooting Tips | Support | Signalway Antibody | Signalway Antibody

What went wrong? A Western Blot Troubleshooting Guide
What went wrong? A Western Blot Troubleshooting Guide

Western Blot Troubleshooting | Antibody Company, Buy Antibodies Online
Western Blot Troubleshooting | Antibody Company, Buy Antibodies Online

Western-Blot - Anti Light-Chain Secondary Antibodies - JIR
Western-Blot - Anti Light-Chain Secondary Antibodies - JIR

Western Blotting Troubleshooting Guide | Cell Signaling Technology
Western Blotting Troubleshooting Guide | Cell Signaling Technology

Western Blot Troubleshooting: What to do about non-specific bands - Azure  Biosystems
Western Blot Troubleshooting: What to do about non-specific bands - Azure Biosystems

Western Blot for P65 (NF-kB) K310 Acetylation Stem Cells Whole Cell Lysate  | Biocompare Antibody Review
Western Blot for P65 (NF-kB) K310 Acetylation Stem Cells Whole Cell Lysate | Biocompare Antibody Review

Western Blotting
Western Blotting

Blocking Buffers for Western Blot and ELISA | Thermo Fisher Scientific - US
Blocking Buffers for Western Blot and ELISA | Thermo Fisher Scientific - US

How Western Blotting Works | Lab Manager
How Western Blotting Works | Lab Manager

PLOS Pathogens: Vpu Binds Directly to Tetherin and Displaces It from  Nascent Virions
PLOS Pathogens: Vpu Binds Directly to Tetherin and Displaces It from Nascent Virions

General Recommendations for Using Secondary Antibodies in Western blots -  Dianova Int.
General Recommendations for Using Secondary Antibodies in Western blots - Dianova Int.

SOLVED: Your Three Most Common Western Blotting Problems
SOLVED: Your Three Most Common Western Blotting Problems

PLOS ONE: Revisiting G3BP1 as a RasGAP Binding Protein: Sensitization of  Tumor Cells to Chemotherapy by the RasGAP 317–326 Sequence Does Not Involve  G3BP1
PLOS ONE: Revisiting G3BP1 as a RasGAP Binding Protein: Sensitization of Tumor Cells to Chemotherapy by the RasGAP 317–326 Sequence Does Not Involve G3BP1

Western blot - Wikipedia
Western blot - Wikipedia

Western Blot for P65 (NF-kB) K310 Acetylation Stem Cells Whole Cell Lysate  | Biocompare Antibody Review
Western Blot for P65 (NF-kB) K310 Acetylation Stem Cells Whole Cell Lysate | Biocompare Antibody Review

Western Blot Detection of Phosphorylation Events - Best Practice | Bio-Rad
Western Blot Detection of Phosphorylation Events - Best Practice | Bio-Rad

Too many bands in western blot for Egr1 protein, what should I do?
Too many bands in western blot for Egr1 protein, what should I do?

I am getting very intense non-specific bands in western blot, but very  faint band of protein of interest. What should i do?
I am getting very intense non-specific bands in western blot, but very faint band of protein of interest. What should i do?

IP-Western | Life Science Research | Merck
IP-Western | Life Science Research | Merck