Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?
![Thermo Scientific™ Pierce™ 10X Western Blot Transfer Buffer, Methanol-free: Buffers Buffers and Solutions | Fisher Scientific Thermo Scientific™ Pierce™ 10X Western Blot Transfer Buffer, Methanol-free: Buffers Buffers and Solutions | Fisher Scientific](https://assets.fishersci.com/TFS-Assets/BID/product-images/00000-Generic-Photo-6x4.jpg-650.jpg)
Thermo Scientific™ Pierce™ 10X Western Blot Transfer Buffer, Methanol-free: Buffers Buffers and Solutions | Fisher Scientific
![After transferring on to a membrane step in western blot, my marker and protein bands are smeared. Why does this happen? After transferring on to a membrane step in western blot, my marker and protein bands are smeared. Why does this happen?](https://www.researchgate.net/profile/Mohamed-Mohamed-44/post/After-transferring-on-to-a-membrane-step-in-western-blot-my-marker-and-protein-bands-are-smeared-Why-does-this-happen/attachment/59d61db96cda7b8083a16c98/AS%3A272138454405120%401441894271882/download/001.jpg)
After transferring on to a membrane step in western blot, my marker and protein bands are smeared. Why does this happen?
![10x Tris/Glycine Buffer for Western Blots and Native Gels #1610734 | Ricerca bio-medica biotecnologie | Bio-Rad 10x Tris/Glycine Buffer for Western Blots and Native Gels #1610734 | Ricerca bio-medica biotecnologie | Bio-Rad](https://www.bio-rad.com/webroot/web/images/lsr/products/electrophoresis/sku_view/global/161-0734_view.jpg)